| 1. | Adsorption kinetics and equilibrium process of protein in hydrophobic interaction chromatography 疏水层析蛋白质动力学与平衡过程的考察 |
| 2. | The forces directing these molecular packings are van der waals forces and hydrophobic interactions i n the hydrocarbon part of the molecule 分子的烃基以范德华力相互作用,极性基间主要是静电引力。 |
| 3. | Supramolecular action is a kind of molecular interaction with molecular recognition ability . it includes van der waals and electrostatic interactions , hydrogen - bonding , - bonding and hydrophobic interaction , which are accompanied by spacial effect 超分子作用是一种具有分子识别能力的分子间相互作用,是空间效应影响下的范德华力、静电引力、氢键力、相互作用与疏水相互作用等。 |
| 4. | Quantitatively describing the principle of protein - protein interactions and recognition in molecular level is the key point for understanding the relationship between structure and function of protein complexes and designing protein complexes , hi these interactions among proteins , electrostatic and hydrophobic interactions are the two most important ones , which let protein molecules bond systematically 在分子水平上定量地阐明蛋白质分子之间的亲合力和相互识别的机制,是正确地认识蛋白质聚集体结构和功能之间的关系并合理地设计出有用的蛋白质分子聚集体的关键。蛋白质分子间相互作用,其中最主要的是静电和疏水相互作用,使蛋白质分子能有序地结合在一起。 |
| 5. | One is a combination of ion exchange chromatography on q sepharose ff , hydrophobic interaction chromatography on phenyl hp , gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q . the other is a combination of ion exchange chromatography on q sepharose ff , two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially 上清液中重组蝗蛇毒类激血酶的纯化是通过设计的不同分离纯化方法组合进行的,并对每一种组合进行了活力与纯度及口收率的评价,结果表明两种组合方式都具有一定的实用性和可操作性。 |
| 6. | The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp . the fermentation liquor also showed good dehairing activity . the alkaline protease ( named dhap , dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography , ion exchange and gel filtration 通过cm - sepharosefastflow离子交换层析, deae - sepharosefastflow离子交换层析, sephacryls - 100 , sephacryls - 200凝胶过滤层析,疏水层析等纯化步骤对短小芽孢杆菌发酵液中的碱性蛋白酶进行了纯化。 |
| 7. | Tem image showed that nanorods form after the modification , which were 50 ~ 100nm in width and several microns in length . the rodlike nanostructures may be caused by the special packing of these ots - coated nanotubes through hydrophobic interaction between the long fatty chains . then the lb technique was attempted to assemble the nanorods 使用lb技术对修饰后得到的纳米棒进行了组装,使用原子力显微镜观察形貌,结果表明纳米棒排列混乱无序且有聚集现象;经二十二烷酸( ba )作为分散剂混合做膜后,纳米棒的分散性良好。 |
| 8. | The central issue of this paper is to review the progress on molecular assembly based on calixarene derivatives , which covers the self - assembly of calixarenes in solution , solid as well as surface through hydrogen bonding , metal - induced force , - interactions , hydrophobic interactions as well as other non - covalently weaker forces 主要综述杯芳烃衍生物通过氢键、金属诱导配位、 -作用、疏水作用等非共价键弱相互作用力在溶液状态、固态和界面的分子自组装方面的研究进展。 |
| 9. | Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth . ba - dfe was purified from the supernatant of b . amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation , ion - exchange chromatography on cm - and deae - sepharose fast flow , hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50 . the purified enzyme displayed thermophilic , hydrophilic and strong fibrinolytic activity 通过硫酸铵分级沉淀、 cm - sepharosefastflow和deae - sepharosefastflow离子交换层析、 phenylsepharose6fastflow疏水层析和sephadexg - 50凝胶过滤等方法,从解淀粉芽孢杆菌dc - 4的发酵液中分离纯化出电泳纯的ba - dfe 。 |